Key Considerations for a Spatial Biology Light Source

The trend of spatial biology is gathering pace, and staying at the forefront of this powerful approach requires imaging platforms that incorporate the latest technologies.

This includes the light source, and high-performance illumination plays a valuable, but sometimes underestimated role in the performance of spatial biology systems.

CoolLED designs and manufactures cutting edge LED Illumination Systems to give manufacturers the edge in competitive and quickly evolving markets such as spatial biology. At CoolLED, we strive to deliver tailored spatial biology light source solutions that redefine illumination standards.

Understanding Spatial Biology Light Sources

Our spatial biology light sources are engineered to provide unparalleled spectral coverage, speed, precision and reliability, empowering researchers to unravel the complexities of cellular dynamics with unprecedented clarity and depth.

  • Spectral Coverage: Wavelengths are available to cover 340 nm to 850 nm, offering compatibility with the vast majority of fluorophores or chromogens.
    Moreover, compatibility of the imaging platform is maximised thanks to the discrete spectral nature of LEDs and ability to include up to twelve individual channels in a single spatial biology light source.
  • Speed: Whether imaging large samples, or many samples (or both), minimising turnaround time is vital. And this is where our LED Illumination Systems can help, with industry-leading <7 µs TTL triggering, combined with powerful illumination enabling the fastest exposure times. Clever Pinkel filter configurations also bypass latency of filter wheels, delivering the fastest imaging speeds available.
  • Homogeneity: Imaging large sample regions relies on imaging stitching. CoolLED Illumination Systems offer highly homogenous illumination across the field of view, avoiding vignetting artefacts which can interfere with analysis of stitched images.  This is especially ideal for quantitative analysis, providing precise results across the whole sample.
  • Precision and Accuracy: Our light sources are meticulously crafted to deliver stable and repeatable illumination over time as well as space, which is vital for ensuring consistent and reproducible results and ideal for quantitative analysis.
  • Reliability and Durability: Built to endure the rigors of daily laboratory use, our light sources are engineered for long-term reliability and durability, providing peace of mind and uninterrupted workflow efficiency.
  • Sustainability: LEDs are famous for their energy efficiency, and CoolLED Illumination Systems not only reduce energy consumption but also offer a long lifetime.
  • Customisable Solutions: The requirements of spatial biology platforms are unique. That’s why we offer the customisable Amora Series which can be tailored to meet the specific requirements and challenges of your spatial biology application. Take a look at Our Technology to see the range of options available.

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    Replacing a Metal Halide or Mercury Lamp?

    Over the last decade, LEDs have become the go-to illumination technology for widefield fluorescence, replacing traditional mercury and metal halide lamps due to enhanced performance, convenience and environmental credentials. For example, utilising LED technology as the spatial biology light source means the illumination remains stable over time, delivering precise experimental results – which is especially important when scanning a whole slide. Moreover, electronic control options such as TTL triggering reach speeds of under 7 µs.

    Some models further enable high-speed imaging with the facility to house single-band excitation filters within the light source. Sitting in front of each LED channel, these allow a Pinkel or Sedat configuration, and in a similar way to full multi-band configurations avoid any latency of mechanical movement in the excitation light path. Coupled with powerful illumination, this allows short exposure times and significantly increases the speed of imaging large areas such as whole slides.

    Partnering with Solid-State OEM Illumination Experts

    At CoolLED, we take pride in our ability to guide our partners and work together to create custom solid-state OEM illumination solutions. We understand that each project requires a unique approach, and our team of experts is dedicated to working closely with you to develop tailored solutions that align with your specific requirements.

    Need a Bespoke Solution? Explore the Amora Series of Spatial Biology Light Sources

    At CoolLED, we offer a comprehensive selection of spatial biology light sources designed to meet the diverse needs and applications of today’s leading manufacturers. Known as the Amora Series, the platform can be customised to serve the most unique requirements.

    The Amora Series takes the strengths of our popular off-the-shelf products and offers the flexibility to choose anywhere between two and twelve LED channels. Additionally, a menu of features around control, spectral coverage and connectivity (see Our Technology) matches a solution to the customer’s requirements. The Amora Series offers two key benefits:

    Product Reliability – CoolLED products are famous for their reliability and have had many years to prove it. They are also backed by CoolLED’s world-renowned support and a long, fully comprehensive warranty.

    Production Speed – Focusing on selected features allows us to create a prototype on much tighter timescales.

    Why Work with CoolLED?

    In the dynamic realm of spatial biology, the significance of the light source cannot be overstated. Since 2006 when CoolLED launched the first LED light source for fluorescence microscopy, we have expanded our expertise to become world leaders in this arena. CoolLED now enables manufacturers of spatial biology platforms to gain a competitive edge through:

    • Cutting-Edge Technology: CoolLED stands at the forefront of innovation, leveraging state-of-the-art LED technology to deliver superior performance and unrivalled flexibility in spatial biology illumination.
    • Trusted Partnership: Our experts are passionate when it comes to pushing the boundaries of science and engineering, and will walk you through every stage of the process as we work together.

    A Simplified Solution

    For some spatial biology platforms, an even more efficient approach is possible by utilising one of CoolLED’s many off the shelf multi-channel LED Illumination Systems. For example, researchers from Roche Diagnostics Solutions (Ventana Medical Systems, Inc.), have utilised the eight channel pE-800 Illumination System for a novel spectroscopic imaging method compatible with existing clinical workflows.1 The combination of high-speed (<7 µs) TTL triggering and eight powerful LED channels was ideal for this application, delivering the optimal spatial biology light source without the need for any further modification.  Their research paper can be found here

    Contact us today to learn more about CoolLED’s spatial biology light sources and unlock the competitive advantage that high-performance LED illumination can deliver. We look forward to being your trusted partner in illuminating the future of spatial biology research.

    Contact Us Today

    Elevate your application with CoolLED’s exceptional OEM illumination solutions. Contact us today to discuss your requirements, explore the Amora Series, and discover the future of lighting with CoolLED – your trusted source for spatial biology light sources.


    1. Rajendran R, Beck RC, Waskasi MM, Kelly BD, Bauer DR. Digital analysis of the prostate tumor microenvironment with high-order chromogenic multiplexing. J Pathol Inform. 2023;15:100352. Published 2023 Nov 21. doi:10.1016/j.jpi.2023.100352

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