Advanced Techniques Using LED Illumination 

There is a whole range of newly emerging techniques that have traditionally employed laser light to illuminate a sample, but are now turning to LEDs. An example is structured illumination microscopy (SIM), a wide-field technique that can improve the lateral resolution...
Turning Faster with LEDs 

Turning Faster with LEDs 

Until recently, spinning disc confocal microscopy systems featured laser illumination due to light loss through the system. However, now that LEDs are more powerful, they are being used in some of the less expensive systems. With discs that permit greater transmission...
Optogenetics – overcoming challenges.

Optogenetics – overcoming challenges.

In neuroscience, there is a long-standing desire to selectively target one cell type for investigation without affecting other cells in the sample. This was achieved, in part, 15 years ago by using Cre-Lox methods of labelling cells that transiently express genes...

Focus Drift Correction with LEDs

LEDs are not just used for illuminating a sample in time-lapse microscopy. They are also used in most focus drift correction systems (from manufacturers such as Leica, Nikon and Zeiss), which are feedback devices used to maintain focus. At high magnifications, the...